Description
Human CASP11 (Caspase 11) ELISA Kit | ELK5519 | Gentaur UK, US & Europe Distribution
Alternative Names: SRSF2IP; SFRS2IP; SIP1; SRRP129; Cysteinyl Aspartate Specific Proteinases 11; Apoptosis-Related Cysteine Peptidase 11; Splicing Factor, Arginine/Serine-Rich 2, Interacting
Species: Human
Assay Type: Sandwich
Sensitivity: 0.059 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal transduction;Apoptosis
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caspase 11 (CASP11) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Caspase 11 (CASP11) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Caspase 11 (CASP11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Caspase 11 (CASP11) in the samples is then determined by comparing the OD of the samples to the standard curve.