Human CD200 (Cluster Of Differentiation 200) ELISA Kit | ELK3548

Human CD200 (Cluster Of Differentiation 200) ELISA Kit | ELK3548 | Gentaur UK, US & Europe Distribution

Alternative Names: MOX1; MOX2; MRC; OX-2; My033; Antigen Identified By Monoclonal Antibody MRC OX-2

Species: Human

Assay Type: Sandwich

Sensitivity: 12.9 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: Serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 200 (CD200) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cluster Of Differentiation 200 (CD200) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 200 (CD200), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cluster Of Differentiation 200 (CD200) in the samples is then determined by comparing the OD of the samples to the standard curve.