Human CDH17 (Cadherin 17) ELISA Kit | ELK2743

Human CDH17 (Cadherin 17) ELISA Kit | ELK2743 | Gentaur UK, US & Europe Distribution

Alternative Names: CDH16; HPT-1; HPT1; LI-Cadherin; Cadherin, Liver Intestine; Intestinal peptide-associated transporter HPT-1; Liver-intestine cadherin

Species: Human

Assay Type: Sandwich

Sensitivity: 14.1 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;Hepatology;Gastroenterology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cadherin 17 (CDH17) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cadherin 17 (CDH17) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cadherin 17 (CDH17), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cadherin 17 (CDH17) in the samples is then determined by comparing the OD of the samples to the standard curve.