Human CDK1 (Cyclin Dependent Kinase 1) ELISA Kit | ELK2253

Human CDK1 (Cyclin Dependent Kinase 1) ELISA Kit | ELK2253 | Gentaur UK, US & Europe Distribution

Alternative Names: CDC2; CDC28A; CDK1; CDC28-A; CDKN1; P34CDC2; p34 protein kinase; Cell Division Control Protein 2 Homolog; Cell Division Cycle 2, G1 To S And G2 To M

Species: Human

Assay Type: Sandwich

Sensitivity: 0.068 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction;Enzyme & Kinase;Tumor immunity;Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin Dependent Kinase 1 (CDK1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cyclin Dependent Kinase 1 (CDK1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclin Dependent Kinase 1 (CDK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclin Dependent Kinase 1 (CDK1) in the samples is then determined by comparing the OD of the samples to the standard curve.