Human Cell division control protein 6 homolog (CDC6) ELISA Kit | KTE62199

Human Cell division control protein 6 homolog (CDC6) ELISA Kit | KTE62199 | Gentaur UK, US & Europe Distribution

Application: This Human Cell division control protein 6 homolog (CDC6) ELISA Kit employs a two-site sandwich ELISA to quantitate CDC6 in samples. An antibody specific for CDC6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCDC6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CDC6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CDC6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Cell division control protein 6 homolog microplate
• Human Cell division control protein 6 homolog standard
• Human Cell division control protein 6 homolog detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Cell division control protein 6 homolog (CDC6) ELISA Kit has high sensitivity and excellent specificity for detection of Human CDC6. No significant cross-reactivity or interference between Human CDC6 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The protein encoded by CDC6 is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins.

Alternative Names: CDC6; CDC18L; HsCDC18; HsCDC6; CDC18 (cell division cycle 18; S.pombe; homolog) -like; CDC6 cell division cycle 6 homolog; CDC6-related protein; cell division cycle 6 protein

Search name: CDC6; CDC18L; HsCDC18; HsCDC6; CDC18 (cell division cycle 18; S.pombe; homolog) -like; CDC6 cell division cycle 6 homolog; CDC6-related protein; cell division cycle 6 protein

Tag: CDC6