Human CellExp™ CD27 Ligand/ CD70, Fc Tag, Human Recombinant | P1366

Cluster of Differentiation 70 (CD70) is also known as CD27 ligand (CD27L / CD27LG), TNFSF7, TNFSF7G, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and is a surface antigen found on activated T-and B-lymphocytes and mature dendritic cells. Binding of CD70 to its receptor CD27 induces in priming, effector functions, differentiation and memory formation of T-cells, and thus is invloved in the biological processes including T-cell activation, the proliferation of costimulated T-cells, as well as the generation of cytolytic T-cells. CD70 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation.

P1366 | Human CellExp CD27 Ligand/ CD70 Fc Tag Human Recombinant DataSheet

Biomolecule/Target: CD70

Synonyms: CD70, CD27LG, TNFSF7, TNFSF7G, CD27L

Alternates names: INT-1, Proto-oncogene Int-1 homolog

Taglines: Cytokine that binds to CD27 and plays role in T-cell activation.

NCBI Gene ID #: 7471

NCBI Gene Symbol: WNT1

Gene Source: Human

Accession #: P04628

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/g by LAL method

Activity (Specifications/test method): Measured by its binding ability in a functional ELISA

Biological activity: The ED was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED for this effect is 1.5 - 2.5 ng/ml in the presence of 200 ng/ml of human BMP-2.

Results: 1.5 - 2.5 ng/m

Binding Capacity: Immobilized Human CD27, Mouse IgG2a Fc Tag at 2 g/mL (100 L/well) can bind Human CD27 Ligand, Fc Tag with a linear range of 0.4-13 ng/mL.

Unit Definition: N/A

Molecular Weight: 38.4 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from PBS

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 µg/µl. The solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A