Human CellExp™ CD28, Human/ Cynomolgus / Rhesus macaque Recombinant | P1471

Human CD28 (Cluster of Differentiation 28) is expressed on the cell surface as a glycosylated, disulfide-linked homodimer. CD28 is the founding member of a subfamily of costimulatory molecules characterized by an extracellular variable immunoglobulin-like domain. Other members of the subfamily include ICOS, CTLA4, PD1, PD1H, and BTLA. CD28 and CTLA4 are highly homologous and compete for the same ligands B7-1 (CD80) and B7-2 (CD86). CD28 is expressed constitutively on almost all human CD4 T cells and approximately 50% of CD8 T cells. CD28 has diverse effects on T cell functions including the induction of cell proliferation and cytokine production and promotion of T-cell survival. Experiments showed that CD28 and CTLA4 have opposing effects on T cell stimulation. CD28 provides an activating signal and CTLA4 provides an inhibitory signal. Translation of a basic understanding of CD28 function into immunomodulatory therapeutics has been uneven, with both successes and failures from pre-clinical studies.

P1471 | Human CellExp™ CD28, Human/ Cynomolgus / Rhesus macaque Recombinant DataSheet

Biomolecule/Target :

Synonyms: T-cell-specific surface glycoprotein CD28, CD antigen: TP44, Tp44, T-Cell-Specific Surface Glycoprotein, CD28 Molecule

Alternates names: T-cell-specific surface glycoprotein CD28, CD antigen: TP44, Tp44, T-Cell-Specific Surface Glycoprotein, CD28 Molecule

Taglines: T-cell-specific surface glycoprotein CD28

NCBI Gene ID #: 940

NCBI Gene Symbol: CD28

Gene Source: Human/ Cynomolgus / Rhesus macaque

Accession #: P10747

Recombinant: True

Source: HEK 293 cells

Purity by SDS-PAGE: >90%

Assay: SDS-PAGE

Purity:

Assay #2:

Endotoxin Level:

Activity (Specifications/test method):

Biological activity: Human biotinylated CD28 can bind to immobilized human CD86 ( Cat# 7496 at 5 ug/ml, 100 ul/well) with a linear detection range of 0.08 to 0.6 ug/ml.

Results:

Binding Capacity:

Unit Definition:

Molecular Weight: This protein is fused with a polyhistidine tag at the C-terminus with the calculated molecular weight of ~16 kDa. The predicted N-terminus is Asn 19 (UniProtKB – P10747 CD28_HUMAN). DTT-reduced protein shows a band about 35-45 kDa due to heavy glycosylation.

Concentration:

Appearance: Lyophilized powder

Physical form description: Lyophilized

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4.

Amino acid sequence: Asn 19 - Pro 152

Handling: Centrifuge the vial prior to opening.

Usage: