Human CellExp™ CD33/SIGLEC-3 | 7370

Myeloid cell surface antigen CD33 also known as SIGLEC3, Siglecs (sialic acid binding Ig-like lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N--terminal Ig--like V--type domain, one Ig--like C2--type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 Domain (s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia.

7370 | Human CellExp CD33 / SIGLEC-3 human recombinant DataSheet

Biomolecule/Target: CD33/SIGLEC-3

Synonyms: CD33, SIGLEC3, gp67

Alternates names: Apolipoprotein E2, apolipoprotein, apolipoproteins

Taglines: Human recombinant with 6xHis tag

NCBI Gene ID #: 348

NCBI Gene Symbol: ApoE

Gene Source: Human

Accession #: NM_000041

Recombinant: Yes

Source: HEK293 cells (Asp 18-His 259 of human protein)

Purity by SDS-PAGEs: 90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/g by LAL method

Activity (Specifications/test method): N/A

Biological activity: Test in process

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 34.3 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 m filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Reconstitute in dHO to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A