Human CellExp™ CD55/DAF, human recombinant | 7432

CD55, also known as DAF or decay accelerating factor, is a member of the RCA (regulators of complement activation) family characterized by four to 30 SCRs (short consensus repeats) in their plasma-exposed regions. CD55 /DAF is a 70 kDa membrane protein that regulates the complement system on the cell surface. It prevents the assembly of the C3bBb complex (the C3-convertase of the alternative pathway) or accelerates the disassembly of preformed convertase, thus blocking the formation of the membrane attack complex. This glycoprotein is broadly distributed among hematopoietic and non-hematopoietic cells. It is a determinant for the Cromer blood group system. CD55 is known to bind CD97 via the first SCR. It also binds physiologically generated C3 convertases with its second and third SCRs. Binding results in an accelerated “decay”, or dissociation of active C3 convertases, thus blocking the development of C’ attack complexes on non-foreign cells. Finally, viruses and bacteria are also known to utilize multiple SCR sites for infection.

7432 | Human CellExp CD55/DAF human recombinant DataSheet

Biomolecule/Target: CD55/DAF

Synonyms: CD55, DAF, CR, CROM, TC

Alternates names: Growth hormone 1, Somatotropin, Pituitary growth hormone

Taglines: A membrane protein that regulates the complement system on the cell surface.

NCBI Gene ID #: 378781

NCBI Gene Symbol: SOMA

Gene Source: Human

Accession #: P08998

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGEs: 99%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/g by LAL method

Activity (Specifications/test method): N/A

Biological activity: GH Chicken Recombinantis fully biologically active in homologous assays and in PDF-P1 3B9 cells stably transfected with rabbit GH receptors.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 22.255 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 m filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile HO or 0.4 % NaHCO adjusted to pH 8-9 to a concentration 100 µg/ml and sterile filtered. This solution can then be diluted into other aqueous buffers, preferably in the presence of carrier protein.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Phe-Pro-Ala