Human CellExp™ Her2/ErbB2, Extracellular Domain (ED), Human Recombinant | P1160

Human Epidermal growth factor Receptor 2 (HER2) is also called ERBB2, HER-2, HER-2 /neu, NEU, NGL, TKR1 and c-erb B2, and is a protein involved in higher aggressiveness of breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. Approximately 30% of breast cancers have an amplification of the HER2 gene or overexpression of its protein product. Overexpression of this receptor in breast cancer is associated with increased disease recurrence and worse prognosis. HER2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation.

P1160 | Human CellExp Her2/ErbB2 Extracellular Domain ED Human Recombinant DataSheet

Biomolecule/Target: ERBB2

Synonyms: erbB-2, MNL 19, CD340, Neu, c-ErbB-2, NEU, NGL, TKR1

Alternates names: Left-right determination factor 1, Left-right determination factor B, Protein lefty-1, Protein lefty-B

Taglines: Involved in the signal transduction pathways leading to cell growth and differentiation.

NCBI Gene ID #: 10637

NCBI Gene Symbol: LEFTY1

Gene Source: Human

Accession #: O75610

Recombinant: Yes

Source: Human 293 cells

Purity by SDS-PAGEs: 90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by its binding ability in a functional ELISA.

Biological activity: The specific activity was determined by dose dependent ability to inhibit BMP-4 (6.5 ng/mL) induction of alkaline phosphatase production in the MC-3T3-E1 cell line (Mouse Chondrogenic cell line).

Results: Typically 10 to 40 ng/mL ED50.

Binding Capacity: Immobilized Human Her2 (ED) at 0.05 g/ml (100 l/well) can bind trastuzumab (Herceptin) with a linear range of 0.05-3 ng/ml.

Unit Definition: N/A

Molecular Weight: 38 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 m filtered solution in PBS pH 7.4. Generally 5-8% Mannitol or Trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Reconstitute in sterile water.

Amino acid sequence: N/A