Human CellExp™ IGF1R/CD221, human recombinant | 7490

The Insulin-like Growth Factor 1 Receptor IGF1Ris also known as CD221, JTK13, and is a transmembrane receptor that is activated by IGF-1 and by the related growth factor IGF-2. It belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. IGF1R is made up of two alpha subunits and two beta subunits. Both the and subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds to form a functional transmembrane chain. The chains are located extracellularly while the subunit spans the membrane and are responsible for intracellular signal transduction upon ligand stimulation. IGF1R have a binding site for ATP, which is used to provide the phosphates for autophosphorylation. There is a 60% homology between IGF1R and the insulin receptor. In response to ligand binding, the chains induce the tyrosine autophosphorylation of the chains. This event triggers a cascade of intracellular signaling that, while somewhat cell type specific, often promotes cell survival and cell proliferation.

7490 | Human CellExp IGF1R/CD221 human recombinant DataSheet

Biomolecule/Target: IGF1R/CD221

Synonyms: IGF-1R, IGF1R, CD221, IGFIR, IGFR, IGFR, JTK13, MGC142170, MGC142172, MGC18216, Insulin-like Growth Factor 1 (IGF-1) Receptor

Alternates names: pS2 protein, HP1.A, Breast Cancer Estrogen-Inducible protein (BCEI), PNR2, Breast cancer estrogen-inducible protein, PNR-2, Polypeptide P1.A, Protein pS2

Taglines: This receptor mediates the effects of IGF-1

NCBI Gene ID #: 7031

NCBI Gene Symbol: TFF1

Gene Source: Human

Accession #: P04155

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/g by LAL method

Activity (Specifications/test method): N/A

Biological activity: Determined by its ability to chemoattract human MCF-7 cells using a concentration 5-10 g/ml.

Results: 5-10 g/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 8.2 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 m filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A