Human CellExp™ IgG2 Fc, Human Recombinant | P1189

Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b. It was found that IgG2a was superior to IgG1 in activating complement. The glycosylation of the circulating immunoglobulin- (IgG) antibody molecules changes in rheumatoid arthritis. Ig gamma-2 chain Fc region contains two constant regions of IgG2 H chain (CH2, CH3).

P1189 | Human CellExp IgG2 Fc Human Recombinant DataSheet

Biomolecule/Target: IGHG2

Synonyms: IgG Fc

Alternates names: Complement Fragments C5a, C5a anaphylatoxin chemotactic receptor

Taglines: Constant region of immunoglobulin heavy chains.

NCBI Gene ID #: 15139

NCBI Gene Symbol: C5a

Gene Source: Mouse

Accession #: NP_034536

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per/g

Activity (Specifications/test method): N/A

Biological activity: ED 1-3 nM as determined by enzyme release assay of myeloperoxidase

Results: 1-3 nmolar

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 9 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 m filtered solution in PBS pH 7.4. Generally 5-8% Mannitol or Trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Reconstitute to a concentration of 1.0 mg/ml in ice-cold HO containing 2.5 mg/ml BSA. The solution should be aliquoted and immediately stored at -70°C.

Amino acid sequence: N/A