Human CellExp™ IL-1 beta / IL-1F2, Human recombinant | P1019

Interleukin-1 beta (IL-1) is also known as catabolin, is a cytokine protein that in humans is encoded by the IL1B gene. IL-1 precursor is cleaved by caspase 1 (interleukin 1 beta convertase). Cytosolic thiol protease cleaves the product to form mature IL-1 beta. IL1 are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL1 and IL1 are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

P1019 | Human CellExp IL-1 beta / IL-1F2 Human recombinant DataSheet

Biomolecule/Target: IL1B

Synonyms: IL1B, IL-1BETA, IL1F2, IL-1, Catabolin

Alternates names: Thymic Stromal Lymphopoietin

Taglines: Potent proinflammatory cytokine

NCBI Gene ID #: 85480

NCBI Gene Symbol: TSLP

Gene Source: Human

Accession #: Q969D9

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1 EU/g

Activity (Specifications/test method): Prolifiration assay

Biological activity: The ED determined by the ability to induce proliferation of human Interleukin-7 receptor & human TSLP Receptor co-transfected with mouse BaF3 cells was found to be less than 0.3 ng/ml.

Results: <0.3 ng/ml.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 15.0 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 m filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Reconstitute in sterile dHO to a concentration of 0.1 -1 mg/ml and let the lyophilized pellet dissolve completely. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A