Human CellExp™ IL-10, Human Recombinant | 6466

The cytokine IL-10 is a key anti-inflammatory mediator with an essential role in the development and proliferation of regulatory T cells, restricting myeloid and chronic inflammatory T cell responses. It has been shown that in solution IL-10 predominantly exists as a homodimer. However, the subunits within the dimer are non-covalently associated, although each subunit contains two intra-chain disulfide bonds. IL10 binds to its hetero-tetrameric receptor comprising IL10RA and IL10RB, which activates multiple pathways that contribute to the downstream anti-inflammatory effects. It also plays important roles in other processes such as sterile wound healing, autoimmunity, cancer, and homeostasis. Given the wide spectrum of the anti-inflammatory properties attributed to IL-10, both IL-10 enhancing and blockade strategies have been tried in a number of diseases and cancer therapy.

6466 | Human CellExp IL-10 Human Recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor.

Alternates names: Thymic Stromal Lymphopoietin

Taglines: An anti-inflammatory cytokine

NCBI Gene ID #: 85480

NCBI Gene Symbol: TSLP

Gene Source: Human

Accession #: Q969D9

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per 1 g of protein

Activity (Specifications/test method): The specific activity was determined by the dose-dependent simulation of the proliferation of MC/9 cells (mouse mast cell line) in the presence of IL-4 using ’s Quick Cell Proliferation colorimetric Assay Kit Plus (Cat. No. K302). The ED50 is typically in a single digit ~ 1ng/ml.

Biological activity: The ED determined by the ability to induce proliferation of human Interleukin-7 receptor & human TSLP Receptor co-transfected with mouse BaF3 cells was found to be less than 0.3 ng/ml.

Results: <0.3 ng/ml.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 15.0 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized

Reconstitution Instructions: Reconstitute in sterile dHO to a concentration of 0.1 -1 mg/ml and let the lyophilized pellet dissolve completely. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A