Human CellExp™ Integrin beta-5 Extracellular Domain (ED), Human Recombinant | P1172

Integrins are a family of cell surface transmembrane receptors, each consisting of an and a subunit that bind to extracellular matrix (ECM) proteins and cellular counter receptors and associate with the cytoskeleton. In mammals, 24 different integrins are generated through different combinations of 18 and 8 subunits. A number of human congenital disorders have been associated with defective integrin-mediated adhesion, including the blistering disorder junctional epidermolysis bullosa, the bleeding disorder Glanzmann’s thrombastenia , leukocyte adhesion deficiency-I (2 integrins in leukocytes) and leukocyte adhesion deficiency-III, nephrotic syndrome and interstitial lung disease and muscular dystrophy. Integrin alpha-V/beta-5 is a receptor for fibronectin and it recognizes the sequence R-G-D in its ligand.

P1172 | Human CellExp Integrin beta-5 Extracellular Domain ED Human Recombinant DataSheet

Biomolecule/Target: ITGB5

Synonyms: ITGB5, integrin subunit beta 5, integrin beta 5

Alternates names: VGR, VGR1, BMP-6, VG-1-related protein

Taglines: Receptor for fibronectin and adenovirus type C

NCBI Gene ID #: 654

NCBI Gene Symbol: BMP6

Gene Source: Human

Accession #: P22004

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: ’s recombinant human BMP-6 is biologically active. The ED as determined by dose-dependent induction of alkaline phosphatase activity with C2C12 cells is in the range of 2-12 µg/ml.

Results: 2-12 µg/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: monomer (15 kDa), dimer (30 kDa)

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 m filtered solution in PBS pH 7.4. Generally 5-8% Mannitol or Trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Reconstitute to a concentration of 0.1-1.0 mg/ml in sterile 20 mM acetic acid. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A