Human CellExp™ LIF, mouse recombinant | 7268

Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.

7268 | Human CellExp LIF mouse recombinant DataSheet

Biomolecule/Target: LIF

Synonyms: LIF, CDF, DIA, HILDA, MLPLI

Alternates names: LIF, CDF, DIA, HILDA, MLPLI

Taglines: Affects cell growth and development

NCBI Gene ID #: 16183

NCBI Gene Symbol: IL2

Gene Source: Mouse

Accession #: P04351

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g) by LAL method

Activity (Specifications/test method): Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.3-1.2 ng/ml

Biological activity: Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.3-1.2 ng/ml

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: Calculated MW of 20.7 kDa with no tags. The predicted N-terminus is Ser 24. DTT-reduced Protein migrates as 33-45 kDa due to different glycosylation.

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.22 micron filter. Lyophilized from 1 x PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Amino acid sequence: N/A