Human CellExp™LRP-5, Fc tag, Human Recombinant | P1457

Low-density lipoprotein receptor-related protein 5(LRP-5) is also known as BMND1, EVR1, EVR4, HBM, LR3, LRP7, OPPG, OPTA1, VBCH2, LDL receptor related protein 5 and PCLD4. LRP5 is a transmembrane low-density lipoprotein receptor that shares a similar structure with LRP6. LRP5 acts as a co-receptor with LRP6 and the Frizzled protein family members for transducing signals by Wnt proteins through the canonical Wnt pathway. This protein plays a key role in skeletal homeostasis. Mutations in LRP5 can lead to considerable changes in bone mass. A loss-of-function mutation causes osteoporosis-pseudoglioma (decrease in bone mass), while a gain-of-function mutation causes drastic increases in bone mass.

P1457 | Human CellExp™LRP-5, Fc tag, Human Recombinant DataSheet

Biomolecule/Target :

Synonyms: LRP-5, LRP-5, LRP-7, LRP7, LR3

Alternates names: LRP-5, LRP-5, LRP-7, LRP7, LR3

Taglines: Frizzled protein family members that plays a key role in skeletal homeostasis

NCBI Gene ID #: 4041

NCBI Gene Symbol: LRP5

Gene Source: Human

Accession #: O75197-1

Recombinant: False

Source: HEK 293 cells

Purity by SDS-PAGE: >90%

Assay: SDS-PAGE

Purity:

Assay #2:

Endotoxin Level: Less than 0.1 EU per ug by the LAL method.

Activity (Specifications/test method):

Biological activity: Immobilized Human Dkk-1, His Tag 5 ug/mL (100 uL/well) can bind Human LRP-5, Mouse IgG2a Fc Tag with a linear range of 0.01-0.313 ug/mL

Results:

Binding Capacity:

Unit Definition:

Molecular Weight: This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 96.6 kDa. The protein migrates as 110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Concentration:

Appearance: Lyophilized

Physical form description: Lyophilized

Reconstitution Instructions: Reconstitute in sterile deionized water to the desired protein concentration.

Amino acid sequence: Glu 644 - Gln 1263

Handling: Centrifuge the vial prior to opening.

Usage: