Human CellExp™ Mesothelin / MSLN, Human Recombinant | P1349

Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.

P1349 | Human CellExp Mesothelin / MSLN Human Recombinant DataSheet

Biomolecule/Target: Mesothelin

Synonyms: MSLN, Mesothelin, MPF, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor

Alternates names: CD14, Cluster of differentiation 14, Human CD14, CD14, h-CD14, rh-CD14, recombinant human CD14, recombinant CD14, CD14, Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

Taglines: Membrane-anchored forms may play a role in cellular adhesion.

NCBI Gene ID #: 12475

NCBI Gene Symbol: CD14

Gene Source: Mouse

Accession #: P10810

Recombinant: Yes

Source: CHO cells

Purity by SDS-PAGEs: 90-95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/g by LAL method

Activity (Specifications/test method): N/A

Biological activity: Inhibits binding of LPS to CD14. Up to 5µg/ml mouse CD14 inhibit binding of FITC-LPS (0.5µg/ml) to mouse CD14+ CHO transfectants

Results: N/A

Binding Capacity: Immobilized Human Mesothelin, His Tag at 0.5 g/mL (100 L/well) can bind Anti-Human MSLN Mab with a linear range of 0.1-2 ng/mL

Unit Definition: N/A

Molecular Weight: 50.0 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 m filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution Instructions: Reconstitute in 10 µl of ultra-pure distilled water. This solution can then be diluted into other aqueous buffers and stored at –20°C or –70°C for future use.

Amino acid sequence: N/A