Human CellExp™ PD-1 / PDCD1 Protein, mouse recombinant | P1086

Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN- by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN- secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.

P1086 | Human CellExp PD-1 / PDCD1 Protein mouse recombinant DataSheet

Biomolecule/Target: PDCD1

Synonyms: PDCD1, PD1, CD279, SLEB2, hPD-1, hPD-l

Alternates names: Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.

Taglines: Inhibits the T-cell proliferation and production of IL-1, IL-4, IL-10 and IFN- by suppressing the activation and transduction of PI3K/AKT pathway.

NCBI Gene ID #: 7422

NCBI Gene Symbol: VEGFA

Gene Source: Human

Accession #: P15692

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1 EU/g

Activity (Specifications/test method): N/A

Biological activity: Determined by the dose-dependent stimulation of the proliferation of human mbilical vein endothelial cells (HUVEC) using a concentration range of 0.1-0.5 ng/ml.

Results: 0.1-0.5 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 28.4 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 m filtered solution in PBS, pH7.4. Generally Mannitol or Trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A