Human CellExp™ PON1, Human Recombinant | P1556

PON1, also known as A esterase1, is a member of the paraoxonase family. It is an enzyme that hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. It is also a major anti-atherosclerotic component of high-density lipoprotein (HDL). This protein is activated by PPAR-gamma, which increases synthesis and release of paraoxonase 1 enzyme from the liver, reducing atherosclerosis. PON1 shows a variety of atheroprotective properties by metabolizing inflammatory lipid peroxides. It has evolved to be a highly promiscuous enzyme capable of hydrolysing a wide variety of substrates such as lactones, cyclic carbonates, organophosphorus pesticides and nerve gases. Recombinant human PON1, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

P1556 | Human CellExp™ PON1, Human Recombinant DataSheet

Biomolecule/Target :

Synonyms: Aromatic esterase 1, A-esterase 1, K-45, Serum aryldialkylphosphatase 1, paraoxonase 1, ESA, MVCD5, PON

Alternates names: Aromatic esterase 1, A-esterase 1, K-45, Serum aryldialkylphosphatase 1, paraoxonase 1, ESA, MVCD5, PON

Taglines: Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides

NCBI Gene ID #: P27169

NCBI Gene Symbol: PON1

Gene Source: Human

Accession #: P27169

Recombinant: FALSE

Source: HEK 293 cells

Purity by SDS-PAGE: >90%

Assay: SDS - PAGE

Purity:

Assay #2:

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Activity (Specifications/test method): Specific activity is > 2,500 pmol/min/ug

Biological activity:

Results:

Binding Capacity:

Unit Definition: One unit is defined as the amount of enzyme that hydrolyze 1pmole of pnitrophenyl acetate to p-nitrophenol per minute at pH7.5 at 37C.

Molecular Weight: 39.0 kDa (His-tag at C-terminus)

Concentration: 0.25 mg/ml (determined by Absorbance at 280nm)

Appearance: Liquid protein

Physical form description: Liquid

Reconstitution Instructions:

Amino acid sequence: 346 aa (16-355 aa)

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans