Human CellExp™ Recombinant EBOV Envelope Glycoprotein 1 | P1060

EBOV encodes seven structural proteins: nucleoprotein (NP), polymerase cofactor (VP35), (VP40), GP, transcription activator (VP30), VP24, and RNA polymerase (L). GP protein contains 160-kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP). GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . GP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. GP2 acts as a class I viral fusion protein. GP1,2 mediates endothelial cell activation and decreases endothelial barrier function. sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha.

P1060 | Human CellExp Recombinant EBOV Envelope Glycoprotein 1 DataSheet

Biomolecule/Target: GP

Synonyms: GP1, GP, Envelope glycoprotein, GP2

Alternates names: Interleukin 31, IL31

Taglines: N/A

NCBI Gene ID #: 76399

NCBI Gene Symbol: IL31

Gene Source: Mouse

Accession #: Q6EAL8

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per/g

Activity (Specifications/test method): N/A

Biological activity: A standard activity has not yet been determined.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 15.7 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 m filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

Reconstitution Instructions: Reconstitute in sterile HO at a concentration of 0.1mg/ml. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage. Reconstituted IL-31 should be stored in working aliquots at -20°C.

Amino acid sequence: MTCSLSFGAP ISKEDLRTTI DLLKQESQDL YNNYSIKQAS GMSADESIQL PCFSLDREAL TNISVIIAHL EKVKVLSENT VDTSWVIRWL TNISCFNPLN LNISVPGNTD ESYDCKVFVL TVLKQFSNCM AELQAKDNTT C