Human CellExp™ sCD14, Human Recombinant | 7122

CD14 is a cell surface anchored glycoprotein that is expressed predominantly by monocytes and tissue macrophages. CD14 associates with MD-2 (LY-96) and TLR4 to form a receptor complex, which signals specifically in response to bacterial lipopolysaccharide (LPS) binding. The CD14/MD-2/TLR4 receptor complex signals via MyD88, TIRAP, and TRAF6, and ultimately activates NF-kappa-. CD14 also exists in a soluble form, designated as sCD14, which is capable of specifically binding LPS in the extracellular space. Recombinant sCD14 is a 331 amino acid glycoprotein comprising the extracellular portion of the CD14 receptor.

7122 | Human CellExp sCD14 Human Recombinant DataSheet

Biomolecule/Target: sCD14

Synonyms: soluble CD14, Cluster of differentiation 14, Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14

Alternates names: PRL-R, hPRLrI, PRL, luteotropic hormone, LTH, PRLR, PRL-R, PRL R

Taglines: A surface glycoprotein involved in signaling in response to bacterial lipopolysaccharide (LPS) binding

NCBI Gene ID #: 5618

NCBI Gene Symbol: PRLR

Gene Source: Human

Accession #: P16471

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): N/A

Biological activity: Determined by the dose-dependent inhibition of Prolactin stimulated proliferation of Nb2 cells and by high affinity binding of ovine Prolactin and other lactogenic hormones in 1:1 molar ratio.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 23.97 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium phosphate, pH 7.5.

Reconstitution Instructions: Reconstitute in sterile dHO to a concentration of 0.1 -1 mg/ml and let the lyophilized pellet dissolve completely. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A