Human CellExp™ Siglec-15, Mouse Recombinant | P1466

Siglec-15 is a DAP12-associated immunoreceptor, which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Siglecs are cell surface proteins that bind sialic acid. They are found primarily on the surface of immune cells and are a subset of the I-type lectins. Siglec-15 consisting of immunoglobulin (Ig)-like domains, transmembrane domain and a short cytoplasmic tail. Siglec-15 is that recognizes sialylated glycans and regulates osteoclast differentiation. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates.

P1466 | Human CellExp™ Siglec-15, Mouse Recombinant DataSheet

Biomolecule/Target :

Synonyms: CD33 antigen-like 3, SIGLEC-15, CD33L3, sialic acid-binding Ig-like lectin 15, Siglec15, Siglec-15

Alternates names: CD33 antigen-like 3, SIGLEC-15, CD33L3, sialic acid-binding Ig-like lectin 15, Siglec15, Siglec-15

Taglines: Transmembrane glycoprotein that controls innate and adoptive immune response.

NCBI Gene ID #: 620235

NCBI Gene Symbol: 620235

Gene Source: Mouse

Accession #: A7E1W8-1

Recombinant: True

Source: HEK 293 cells

Purity by SDS-PAGE: >90%

Assay: SDS-PAGE

Purity:

Assay #2:

Endotoxin Level: Less than 1.0 EU per ug by the LAL method.

Activity (Specifications/test method):

Biological activity: Immobilized Mouse Siglec-15 at 5 ug/mL (100 uL/well) can bind Mouse Human chimeric MAb with a linear range of 0.3-2 ng/mL

Results:

Binding Capacity:

Unit Definition:

Molecular Weight: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 27.5 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Concentration:

Appearance: Lyophilized powder

Physical form description: Lyophilized

Reconstitution Instructions: Reconstitute in sterile deionized water to the desired protein concentration.

Amino acid sequence: Arg 24 - Thr 262

Handling: Centrifuge the vial prior to opening.

Usage: