855

Human CFHR3 (Complement Factor H Related Protein 3) ELISA Kit | ELK6306

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SKU:
855-ELK6306-GEN
$2,058.00 - $2,464.00

Description

Human CFHR3 (Complement Factor H Related Protein 3) ELISA Kit | ELK6306 | Gentaur UK, US & Europe Distribution

Alternative Names: CFHL3; DOWN16; FHR-3; FHR3; HLF4; H factor-like protein 3

Species: Human

Assay Type: Sandwich

Sensitivity: 0.075 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Hematology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Factor H Related Protein 3 (CFHR3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Complement Factor H Related Protein 3 (CFHR3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Factor H Related Protein 3 (CFHR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Factor H Related Protein 3 (CFHR3) in the samples is then determined by comparing the OD of the samples to the standard curve.

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