Human CGa (Chorionic Gonadotropin Alpha Polypeptide) ELISA Kit | ELK5082

Human CGa (Chorionic Gonadotropin Alpha Polypeptide) ELISA Kit | ELK5082 | Gentaur UK, US & Europe Distribution

Alternative Names: FSHA; GPHA1; GPHa; HCG; LHA; TSHA; Thyroid Stimulating Hormone Alpha; Follicle-Stimulating Hormone Alpha Subunit; Luteinizing hormone alpha chain; Lutropin alpha chain

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 49.4 pg/mL

Standard: 10000 pg/mL

Detection Range: 156.25-10000 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Tumor immunity;Endocrinology;Reproductive science;Genetic science;Hormone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chorionic Gonadotropin Alpha Polypeptide (CGa) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chorionic Gonadotropin Alpha Polypeptide (CGa) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chorionic Gonadotropin Alpha Polypeptide (CGa) in the samples is then determined by comparing the OD of the samples to the standard curve.