Human CGb (Chorionic Gonadotropin Beta Polypeptide) ELISA Kit | ELK5460

Human CGb (Chorionic Gonadotropin Beta Polypeptide) ELISA Kit | ELK5460 | Gentaur UK, US & Europe Distribution

Alternative Names: CG-B; CGB3; hCGB; HCG; CGb5; CGb7; CGb8; Choriogonadotropin subunit beta

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 4.64 ng/mL

Standard: 1000 ng/mL

Detection Range: 15.63-1000 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Reproductive science

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chorionic Gonadotropin Beta Polypeptide (CGb) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chorionic Gonadotropin Beta Polypeptide (CGb) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chorionic Gonadotropin Beta Polypeptide (CGb) in the samples is then determined by comparing the OD of the samples to the standard curve.