Human Chymase (CMA1) ELISA Kit | KTE62195

Human Chymase (CMA1) ELISA Kit | KTE62195 | Gentaur UK, US & Europe Distribution

Application: This Human Chymase (CMA1) ELISA Kit employs a two-site sandwich ELISA to quantitate CMA1 in samples. An antibody specific for CMA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCMA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CMA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CMA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Chymase microplate
• Human Chymase standard
• Human Chymase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Chymase (CMA1) ELISA Kit has high sensitivity and excellent specificity for detection of Human CMA1. No significant cross-reactivity or interference between Human CMA1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: CMA1 encodes a chymotryptic serine proteinase that belongs to the peptidase family S1. It is expressed in mast cells and is thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II. Alternative splicing results in multiple variants.

Alternative Names: CMA1; CYH; MCT1; MGC119890; MGC119891; chymase; chymase 1 preproprotein transcript E; chymase 1 preproprotein transcript I; chymase; heart; chymase; mast cell; mast cell protease I

Search name: CMA1; CYH; MCT1; MGC119890; MGC119891; chymase; chymase 1 preproprotein transcript E; chymase 1 preproprotein transcript I; chymase; heart; chymase; mast cell; mast cell protease I

Tag: CMA1