Human Circulating Histone H3 ELISA Kit | ELK8743

Human Circulating Histone H3 ELISA Kit | ELK8743 | Gentaur UK, US & Europe Distribution

Alternative Names: Circulating Histone H3

Species: Human

Assay Type: Sandwich

Sensitivity: 0.62 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Infection immunity;Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Circulating Histone H3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Circulating Histone H3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Circulating Histone H3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Circulating Histone H3 in the samples is then determined by comparing the OD of the samples to the standard curve.