Human CK-18 (Cytokeratin 18) ELISA Kit | ELK8576

Human CK-18 (Cytokeratin 18) ELISA Kit | ELK8576 | Gentaur UK, US & Europe Distribution

Alternative Names: Cell proliferation inducing gene 46 protein ; Cell proliferation inducing protein 46 ; Cell proliferation-inducing gene 46 protein ; CK 18 ; CK-18 ; CK18 ; CYK 18 ; CYK18 ; Cytokeratin 18 ; Cytokeratin endo B ; Cytokeratin-18 ; K 18 ; K18 ; K1C18_HUMAN ; KA18 ; Keratin 18 ; Keratin 18, type I ; Keratin D ; keratin, type I cytoskeletal 18 ; Keratin-18 ; Krt18

Species: Human

Assay Type: Sandwich

Sensitivity: 0.058 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cancer of the liver; Viral hepatitis

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytokeratin 18 (CK-18) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cytokeratin 18 (CK-18) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytokeratin 18 (CK-18), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytokeratin 18 (CK-18) in the samples is then determined by comparing the OD of the samples to the standard curve.