855
Human CLIP (Corticotropin Like Intermediate Lobe Peptide) ELISA Kit | ELK4253
- SKU:
- 855-ELK4253-GEN
Description
Human CLIP (Corticotropin Like Intermediate Lobe Peptide) ELISA Kit | ELK4253 | Gentaur UK, US & Europe Distribution
Alternative Names: CLIP
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 4.89 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Lenght: 2h
Research Area: Endocrinology;Hormone metabolism;Urology
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Corticotropin Like Intermediate Lobe Peptide (CLIP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Corticotropin Like Intermediate Lobe Peptide (CLIP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Corticotropin Like Intermediate Lobe Peptide (CLIP) in the samples is then determined by comparing the OD of the samples to the standard curve.