Human Coagulation factor IX (F9) ELISA Kit | KTE61940

Human Coagulation factor IX (F9) ELISA Kit | KTE61940 | Gentaur UK, US & Europe Distribution

Application: This Human Coagulation factor IX (F9) ELISA Kit employs a two-site sandwich ELISA to quantitate F9 in samples. An antibody specific for F9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyF9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for F9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of F9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Coagulation factor IX microplate
• Human Coagulation factor IX standard
• Human Coagulation factor IX detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Coagulation factor IX (F9) ELISA Kit has high sensitivity and excellent specificity for detection of Human F9. No significant cross-reactivity or interference between Human F9 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: F9 encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of F9, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing.

Alternative Names: F9; RP6-88D7.1; FIX; HEMB; MGC129641; MGC129642; PTC; Christmas factor; factor 9; plasma thromboplastic component

Search name: F9; RP6-88D7.1; FIX; HEMB; MGC129641; MGC129642; PTC; Christmas factor; factor 9; plasma thromboplastic component

Tag: F9