Human COL4a1 (Collagen Type IV Alpha 1) ELISA Kit | ELK2838

Human COL4a1 (Collagen Type IV Alpha 1) ELISA Kit | ELK2838 | Gentaur UK, US & Europe Distribution

Alternative Names: COL4-A1; Arresten; Collagen Alpha-1 (IV) chain

Species: Human

Assay Type: Sandwich

Sensitivity: 1.8 ng/mL

Standard: 300 ng/mL

Detection Range: 4.69-300 ng/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type IV Alpha 1 (COL4a1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Collagen Type IV Alpha 1 (COL4a1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type IV Alpha 1 (COL4a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type IV Alpha 1 (COL4a1) in the samples is then determined by comparing the OD of the samples to the standard curve.