Human Collagen alpha-1 (VI) chain (COL6A1) ELISA Kit | KTE62278

Human Collagen alpha-1 (VI) chain (COL6A1) ELISA Kit | KTE62278 | Gentaur UK, US & Europe Distribution

Application: This Human Collagen alpha-1 (VI) chain (COL6A1) ELISA Kit employs a two-site sandwich ELISA to quantitate COL6A1 in samples. An antibody specific for COL6A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCOL6A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for COL6A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of COL6A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Collagen alpha-1 microplate
• Human Collagen alpha-1 standard
• Human Collagen alpha-1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Collagen alpha-1 (VI) chain (COL6A1) ELISA Kit has high sensitivity and excellent specificity for detection of Human COL6A1. No significant cross-reactivity or interference between Human COL6A1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple-helical domain as their common structural element. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha1 (VI), alpha2 (VI), and alpha3 (VI) chains. The alpha2 (VI) and alpha3 (VI) chains are encoded by the COL6A2 and COL6A3 genes, respectively. The protein encoded by this gene is the alpha 1 subunit of type VI collagen (alpha1 (VI) chain) . Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy.Defects in COL6A1 are a cause of Bethlem myopathy (BM) .

Alternative Names: COL6A1; OPLL; alpha 1 (VI) chain (61 AA) ; collagen VI; alpha-1 polypeptide

Search name: COL6A1; OPLL; alpha 1 (VI) chain (61 AA) ; collagen VI; alpha-1 polypeptide

Tag: COL6A1