Human Complement C2 (C2) ELISA Kit | AE61614HU

Human Complement C2 (C2) ELISA Kit | AE61614HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: C2

Alternative Name: DADB-122G4.1; CO2; DKFZp779M0311; C3/C5 convertase|complement component C2

Application: ELISA

Range: 0.625-40 ng/mL

Sensitivity: 0.291 ng/mL

Intra-Assay: ≤5.2%

Inter-Assay: ≤8.0%

Recovery: 1, 04

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate C2 in samples. An antibody specific for C2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Component C2 is a serum glycoprotein that functions as part of the classical pathway of the complement system. Activated C1 cleaves C2 into C2a and C2b. The serine proteinase C2a then combines with complement factor 4b to create the C3 or C5 convertase. Deficiency of C2 has been reported to associated with certain autoimmune diseases and SNPs in this gene have been associated with altered susceptibility to age-related macular degeneration. This gene localizes within the class III region of the MHC on the short arm of chromosome 6. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described in publications but their full-length sequence has not been determined.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .