Human Coxsackie virus IgM (Cox V-IgM) ELISA Kit | AE59027HU

Human Coxsackie virus IgM (Cox V-IgM) ELISA Kit | AE59027HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: Cox VIgM

Alternative Name: N/A

Application: ELISA

Range: 31.25-2000 ng/mL

Sensitivity: 13.2 ng/mL

Intra-Assay: ≤6.4%

Inter-Assay: ≤10.4%

Recovery: 1, 07

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate Cox VIgM in samples. An antibody specific for Cox VIgM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCox VIgM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Cox VIgM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Cox VIgM bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Immunoglobulin M, or IgM for short, is a basic antibody that is present on B cells. It is the primary antibody against A and B antigens on red blood cells. IgM is by far the physically largest antibody in the human circulatory system. It is produced after an animal has been exposed to an antigen for an extended time or when an animal is exposed to an antigen for the second time. IgM forms polymers where multiple immunoglobulins are covalently linked together with disulfide bonds, mostly as a pentamer but also as a hexamer. IgM has a molecular mass of approximately 900 kD (in its pentamer form) . Because each monomer has two antigen binding sites, a pentameric IgM has 10 binding sites. Typically, however, IgM cannot bind 10 antigens at the same time because the large size of most antigens hinders binding to nearby sites.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .