Human CTXI (Cross-linked Carboxy-terminal telopeptide of type Ⅰ collagen) ELISA Kit | ELK8623

Human CTXI (Cross-linked Carboxy-terminal telopeptide of type Ⅰ collagen) ELISA Kit | ELK8623 | Gentaur UK, US & Europe Distribution

Alternative Names: Cross-linked Carboxy-terminal telopeptide of type 1 collagen; CTX-1; Cross-linked Carboxy-terminal telopeptide of type I collagen; CTX-I; Cross linked C terminal Telopeptides of type I collagen

Species: Human

Assay Type: Sandwich

Sensitivity: 0.266 ng/mL

Standard: 40 ng/mL

Detection Range: 0.63-40 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Bone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cross-linked Carboxy-terminal telopeptide of type Ⅰ collagen (CTXI) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cross-linked Carboxy-terminal telopeptide of type Ⅰ collagen (CTXI) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cross-linked Carboxy-terminal telopeptide of type Ⅰ collagen (CTXI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cross-linked Carboxy-terminal telopeptide of type Ⅰ collagen (CTXI) in the samples is then determined by comparing the OD of the samples to the standard curve.