Human CYSLTR1 (Cysteinyl Leukotriene Receptor 1) ELISA Kit | ELK3943

Human CYSLTR1 (Cysteinyl Leukotriene Receptor 1) ELISA Kit | ELK3943 | Gentaur UK, US & Europe Distribution

Alternative Names: CYSLT1R; CYSLTR; HG55; HMTMF81; Cysteinyl leukotriene D4 receptor; G-protein coupled receptor HG55

Species: Human

Assay Type: Sandwich

Sensitivity: 0.135 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Infection immunity;Endocrinology;Hormone metabolism;Bone metabolism;Rheumatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cysteinyl Leukotriene Receptor 1 (CYSLTR1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cysteinyl Leukotriene Receptor 1 (CYSLTR1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cysteinyl Leukotriene Receptor 1 (CYSLTR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cysteinyl Leukotriene Receptor 1 (CYSLTR1) in the samples is then determined by comparing the OD of the samples to the standard curve.