Human Cytochrome P450 3A5 (CYP3A5) ELISA Kit | KTE62158

Human Cytochrome P450 3A5 (CYP3A5) ELISA Kit | KTE62158 | Gentaur UK, US & Europe Distribution

Application: This Human Cytochrome P450 3A5 (CYP3A5) ELISA Kit employs a two-site sandwich ELISA to quantitate CYP3A5 in samples. An antibody specific for CYP3A5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCYP3A5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP3A5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP3A5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Cytochrome P450 3A5 microplate
• Human Cytochrome P450 3A5 standard
• Human Cytochrome P450 3A5 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Cytochrome P450 3A5 (CYP3A5) ELISA Kit has high sensitivity and excellent specificity for detection of Human CYP3A5. No significant cross-reactivity or interference between Human CYP3A5 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: CYP3A5 encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The encoded protein metabolizes drugs as well as the steroid hormones testosterone and progesterone. CYP3A5 is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Two pseudogenes of CYP3A5 have been identified within this cluster on chromosome 7. Expression of this gene is widely variable among populations, and a single nucleotide polymorphism that affects transcript splicing has been associated with susceptibility to hypertensions. Alternative splicing results in multiple transcript variants.

Alternative Names: CYP3A5; CP35; P450PCN3; PCN3; aryl hydrocarbon hydroxylase; cytochrome P-450; cytochrome P450; subfamilyIA (niphedipine oxidase) ; polypeptide 5; flavoprotein-linked monooxygenase; microsomal monooxygenase; ni

Search name: CYP3A5; CP35; P450PCN3; PCN3; aryl hydrocarbon hydroxylase; cytochrome P-450; cytochrome P450; subfamilyIA (niphedipine oxidase) ; polypeptide 5; flavoprotein-linked monooxygenase; microsomal monooxygenase; ni

Tag: CYP3A5