Human DEFb1 (Defensin Beta 1) ELISA Kit | ELK2711

Human DEFb1 (Defensin Beta 1) ELISA Kit | ELK2711 | Gentaur UK, US & Europe Distribution

Alternative Names: B-DF1; DEFB1; BD1; DEF-B1; DEFB101; HBD1

Species: Human

Assay Type: Sandwich

Sensitivity: 0.138 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Infection immunity;Hepatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 1 (DEFb1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Defensin Beta 1 (DEFb1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 1 (DEFb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Defensin Beta 1 (DEFb1) in the samples is then determined by comparing the OD of the samples to the standard curve.