Human Delta (3, 5) -Delta (2, 4) -dienoyl-CoA isomerase, mitochondrial (ECH1) ELISA Kit | KTE61957

Human Delta (3, 5) -Delta (2, 4) -dienoyl-CoA isomerase, mitochondrial (ECH1) ELISA Kit | KTE61957 | Gentaur UK, US & Europe Distribution

Application: This Human Delta (3, 5) -Delta (2, 4) -dienoyl-CoA isomerase, mitochondrial (ECH1) ELISA Kit employs a two-site sandwich ELISA to quantitate ECH1 in samples. An antibody specific for ECH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyECH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ECH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ECH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Delta microplate
• Human Delta standard
• Human Delta detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Delta (3, 5) -Delta (2, 4) -dienoyl-CoA isomerase, mitochondrial (ECH1) ELISA Kit has high sensitivity and excellent specificity for detection of Human ECH1. No significant cross-reactivity or interference between Human ECH1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Delta (3, 5) -Delta (2, 4) -dienoyl-CoA isomerase, mitochondrial is a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-CoA hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, Δ3, 5-Δ2, 4-dienoyl-CoA isomerase, contains a C-terminal peroxisomal targeting sequence and localizes to peroxisomes. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans, 5-cis-dienoyl-CoA to 2-trans, 4-trans-dienoyl-CoA, indicating that it is a delta3, 5-delta2, 4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.

Alternative Names: ECH1; HPXEL; delta3;5-delta2;4-dienoyl-CoA isomerase; dienoyl-CoA isomerase; peroxisomal enoyl-CoA hydratase 1; peroxisomal enoyl-coenzyme A hydratase-like protein

Search name: ECH1; HPXEL; delta3;5-delta2;4-dienoyl-CoA isomerase; dienoyl-CoA isomerase; peroxisomal enoyl-CoA hydratase 1; peroxisomal enoyl-coenzyme A hydratase-like protein

Tag: ECH1