Human Diacyl glycerol (DAG/DG) ELISA Kit | KTE62147

Human Diacyl glycerol (DAG/DG) ELISA Kit | KTE62147 | Gentaur UK, US & Europe Distribution

Application: This Human Diacyl glycerol (DAG/DG) ELISA Kit employs a two-site sandwich ELISA to quantitate DAG/DG in samples. An antibody specific for DAG/DG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDAG/DG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DAG/DG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DAG/DG bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Diacyl glycerol microplate
• Human Diacyl glycerol standard
• Human Diacyl glycerol detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Diacyl glycerol (DAG/DG) ELISA Kit has high sensitivity and excellent specificity for detection of Human DAG/DG. No significant cross-reactivity or interference between Human DAG/DG and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: A diglyceride, or diacylglycerol (DAG), is a glyceride consisting of two fatty acid chains covalently bonded to a glycerol molecule through ester linkages. Two possible forms exist, 1, 2-diacylglycerols and 1, 3-diacylglycerols. DAGs can acts as surfactants and are commonly used as emulsifiers in processed foods. DAG-enriched oil (particularly 1, 3-DAG) has been investigated extensively as a fat substitute due to its ability to suppress the accumulation of body fat; with total annual sales of approximately USD 200 million in Japan since its introduction in the late 1990s till 2009.

Alternative Names: DAG/DG

Search name: DAG/DG

Tag: DAG/DG