Human Disks large homolog 5 (DLG5) ELISA Kit | KTE62026

Human Disks large homolog 5 (DLG5) ELISA Kit | KTE62026 | Gentaur UK, US & Europe Distribution

Application: This Human Disks large homolog 5 (DLG5) ELISA Kit employs a two-site sandwich ELISA to quantitate DLG5 in samples. An antibody specific for DLG5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDLG5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLG5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLG5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Disks large homolog 5 microplate
• Human Disks large homolog 5 standard
• Human Disks large homolog 5 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Disks large homolog 5 (DLG5) ELISA Kit has high sensitivity and excellent specificity for detection of Human DLG5. No significant cross-reactivity or interference between Human DLG5 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: DLG5 encodes a member of the family of discs large (DLG) homologs, a subset of the membrane-associated guanylate kinase (MAGUK) superfamily. The MAGUK proteins are composed of a catalytically inactive guanylate kinase domain, in addition to PDZ and SH3 domains, and are thought to function as scaffolding molecules at sites of cell-cell contact. The protein encoded by this gene localizes to the plasma membrane and cytoplasm, and interacts with components of adherens junctions and the cytoskeleton. It is proposed to function in the transmission of extracellular signals to the cytoskeleton and in the maintenance of epithelial cell structure. Alternative splice variants have been described but their biological nature has not been determined.

Alternative Names: DLG5; RP13-39P12.4; KIAA0583; LP-DLG; P-DLG5; PDLG; discs large homolog 5; discs large protein LP-DLG; large type of P-DLG; placenta and prostate DLG

Search name: DLG5; RP13-39P12.4; KIAA0583; LP-DLG; P-DLG5; PDLG; discs large homolog 5; discs large protein LP-DLG; large type of P-DLG; placenta and prostate DLG

Tag: DLG5