Human DNA-directed RNA polymerase II subunit RPB11-b1 (POLR2J2) ELISA Kit | AE60078HU

Human DNA-directed RNA polymerase II subunit RPB11-b1 (POLR2J2) ELISA Kit | AE60078HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: POLR2J2

Alternative Name: HRPB11B; MGC105050; MGC54043; RPB11b1; DNA directed RNA polymerase II polypeptide J-related

Application: ELISA

Range: Request Information

Sensitivity: Request Information

Intra-Assay: ≤6.5%

Inter-Assay: ≤11.9%

Recovery: 0, 97

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate POLR2J2 in samples. An antibody specific for POLR2J2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOLR2J2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POLR2J2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POLR2J2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: POLR2J2 is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD) . Consequently, it is not known if this locus expresses a protein or proteins in vivo.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .