Human DNA-directed RNA polymerase II subunit RPB11-b1 (POLR2J2) ELISA Kit | KTE62352

Human DNA-directed RNA polymerase II subunit RPB11-b1 (POLR2J2) ELISA Kit | KTE62352 | Gentaur UK, US & Europe Distribution

Application: This Human DNA-directed RNA polymerase II subunit RPB11-b1 (POLR2J2) ELISA Kit employs a two-site sandwich ELISA to quantitate POLR2J2 in samples. An antibody specific for POLR2J2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOLR2J2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POLR2J2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POLR2J2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human DNA-directed RNA polymerase II subunit RPB11-b1 microplate
• Human DNA-directed RNA polymerase II subunit RPB11-b1 standard
• Human DNA-directed RNA polymerase II subunit RPB11-b1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human DNA-directed RNA polymerase II subunit RPB11-b1 (POLR2J2) ELISA Kit has high sensitivity and excellent specificity for detection of Human POLR2J2. No significant cross-reactivity or interference between Human POLR2J2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: POLR2J2 is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD) . Consequently, it is not known if this locus expresses a protein or proteins in vivo.

Alternative Names: POLR2J2; HRPB11B; MGC105050; MGC54043; RPB11b1; DNA directed RNA polymerase polypeptide J-related

Search name: POLR2J2; HRPB11B; MGC105050; MGC54043; RPB11b1; DNA directed RNA polymerase polypeptide J-related

Tag: POLR2J2