Human DNA2-like helicase (DNA2) ELISA Kit | KTE62003

Human DNA2-like helicase (DNA2) ELISA Kit | KTE62003 | Gentaur UK, US & Europe Distribution

Application: This Human DNA2-like helicase (DNA2) ELISA Kit employs a two-site sandwich ELISA to quantitate DNA2 in samples. An antibody specific for DNA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDNA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DNA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human DNA2-like helicase microplate
• Human DNA2-like helicase standard
• Human DNA2-like helicase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human DNA2-like helicase (DNA2) ELISA Kit has high sensitivity and excellent specificity for detection of Human DNA2. No significant cross-reactivity or interference between Human DNA2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: DNA2 interacted with mitochondrial DNA polymerase-gamma and significantly stimulated its polymerase activity. DNA2 and flap endonuclease-1 (FEN1) synergistically processed intermediate 5-prime flap structures occurring in DNA replication and long-patch base excision repair (LP-BER) in mitochondria. The deduced 1, 076-amino acid protein has a putative ATP/GTP-binding site motif A and a predicted transmembrane domain. Northern blot analysis detected low expression in KG-1 and HeLa cells and in thymus, but not in any other human tissue examined.Yeast Dna2 helicase is a DNA-dependent ATPase that unwinds duplex DNA to generate single-stranded DNA, which then acts as a template for DNA polymerization during DNA replication.

Alternative Names: DNA2; DNA2L; FLJ10063; KIAA0083; MGC133297; DNA replication helicase 2 homolog; DNA2 (DNA replication helicase; yeast; homolog) -like; DNA2 DNA replication helicase 2-like

Search name: DNA2; DNA2L; FLJ10063; KIAA0083; MGC133297; DNA replication helicase 2 homolog; DNA2 (DNA replication helicase; yeast; homolog) -like; DNA2 DNA replication helicase 2-like

Tag: DNA2