Human DnaJ homolog subfamily C member 12 (DNAJC12) ELISA Kit | KTE62000

Human DnaJ homolog subfamily C member 12 (DNAJC12) ELISA Kit | KTE62000 | Gentaur UK, US & Europe Distribution

Application: This Human DnaJ homolog subfamily C member 12 (DNAJC12) ELISA Kit employs a two-site sandwich ELISA to quantitate DNAJC12 in samples. An antibody specific for DNAJC12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDNAJC12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DNAJC12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNAJC12 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human DnaJ homolog subfamily C member 12 microplate
• Human DnaJ homolog subfamily C member 12 standard
• Human DnaJ homolog subfamily C member 12 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human DnaJ homolog subfamily C member 12 (DNAJC12) ELISA Kit has high sensitivity and excellent specificity for detection of Human DNAJC12. No significant cross-reactivity or interference between Human DNAJC12 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Highly conserved C terminus, but lack the G/F-rich and CRR domains. Hahn et al. (1999) identified a J domain-containing protein in mouse and Drosophila. By database searching for sequences homologous to these proteins, Lee et al. (2000) identified a human homolog, which they designated JDP1, that has 2 isoforms generated by alternative use of polyadenylation sites. The primary isoform is 1.2 kb long and encodes a 198-amino acid protein; the other is 0.7 kb long and encodes a 107-amino acid protein. The 198-amino acid protein shares approximately 77% sequence identity with the mouse protein. Northern blot analysis detected a single transcript of 1.3 kb at high levels in brain, heart, and testis, and at reduced levels in kidney and stomach. In mouse and Drosophila, expression is highest in kidney and restricted to heads, respectively.

Alternative Names: DNAJC12; RP11-57G10.2; JDP1; J domain containing protein 1; J domain containing protein 1 (JDP1) ; J domain protein 1

Search name: DNAJC12; RP11-57G10.2; JDP1; J domain containing protein 1; J domain containing protein 1 (JDP1) ; J domain protein 1

Tag: DNAJC12