Human DOT1L (DOT1 Like, Histone H3 Methyltransferase) ELISA Kit | ELK7502

Human DOT1L (DOT1 Like, Histone H3 Methyltransferase) ELISA Kit | ELK7502 | Gentaur UK, US & Europe Distribution

Alternative Names: DOT1; KMT4; Histone-lysine N-methyltransferase, H3 lysine-79 specific; Histone H3-K79 methyltransferase; Lysine N-methyltransferase 4

Species: Human

Assay Type: Sandwich

Sensitivity: 0.058 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DOT1 Like, Histone H3 Methyltransferase (DOT1L) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DOT1 Like, Histone H3 Methyltransferase (DOT1L) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DOT1 Like, Histone H3 Methyltransferase (DOT1L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DOT1 Like, Histone H3 Methyltransferase (DOT1L) in the samples is then determined by comparing the OD of the samples to the standard curve.