Human Doublesex- and mab-3-related transcription factor 2 (DMRT2) ELISA Kit | KTE62011

Human Doublesex- and mab-3-related transcription factor 2 (DMRT2) ELISA Kit | KTE62011 | Gentaur UK, US & Europe Distribution

Application: This Human Doublesex- and mab-3-related transcription factor 2 (DMRT2) ELISA Kit employs a two-site sandwich ELISA to quantitate DMRT2 in samples. An antibody specific for DMRT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMRT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMRT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMRT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Doublesex- and mab-3-related transcription factor 2 microplate
• Human Doublesex- and mab-3-related transcription factor 2 standard
• Human Doublesex- and mab-3-related transcription factor 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Doublesex- and mab-3-related transcription factor 2 (DMRT2) ELISA Kit has high sensitivity and excellent specificity for detection of Human DMRT2. No significant cross-reactivity or interference between Human DMRT2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Raymond et al. (1999) sought other genes with DM domains within the minimal sex reversal region of 9p. Using a degenerate PCR strategy, they isolated a cDNA clone encoding a protein with a DM domain similar to that of DMRT1 from a human adult testis cDNA library. This cDNA, DMRT2, encodes a 226-amino acid peptide sharing 80% amino acid identity with DMRT1 in the core region of the DM domain. The 2 proteins also have an additional 29-amino acid conserved DM domain region.The authors concluded that the results provide further support for the presence of a sex-determining gene (s) on distal 9p and favor the possibility of DMRT1 and/or DMRT2 being the sex-determining gene (s) .

Alternative Names: DMRT2; OTTHUMP00000020964; OTTHUMP00000020966; OTTHUMP00000020967; terra-like protein

Search name: DMRT2; OTTHUMP00000020964; OTTHUMP00000020966; OTTHUMP00000020967; terra-like protein

Tag: DMRT2