Human Dual Specificity Plaosplaatase (DUSP1) ELISA Kit | KTE61969

Human Dual Specificity Plaosplaatase (DUSP1) ELISA Kit | KTE61969 | Gentaur UK, US & Europe Distribution

Application: This Human Dual Specificity Plaosplaatase (DUSP1) ELISA Kit employs a two-site sandwich ELISA to quantitate DUSP1 in samples. An antibody specific for DUSP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDUSP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DUSP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DUSP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Dual Specificity Plaosplaatase microplate
• Human Dual Specificity Plaosplaatase standard
• Human Dual Specificity Plaosplaatase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Dual Specificity Plaosplaatase (DUSP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human DUSP1. No significant cross-reactivity or interference between Human DUSP1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Dual specificity protein phosphatase 1 is an enzymeinduced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes.

Alternative Names: DUSP1; CL100; HVH1; MKP-1; MKP1; PTPN10; serine/threonine specific protein phosphatase

Search name: DUSP1; CL100; HVH1; MKP-1; MKP1; PTPN10; serine/threonine specific protein phosphatase

Tag: DUSP1