Human E3 ubiquitin-protein ligase MYLIP (MYLIP) ELISA Kit | KTE61436

Human E3 ubiquitin-protein ligase MYLIP (MYLIP) ELISA Kit | KTE61436 | Gentaur UK, US & Europe Distribution

Application: This Human E3 ubiquitin-protein ligase MYLIP (MYLIP) ELISA Kit employs a two-site sandwich ELISA to quantitate MYLIP in samples. An antibody specific for MYLIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYLIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYLIP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYLIP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human E3 ubiquitin-protein ligase MYLIP microplate
• Human E3 ubiquitin-protein ligase MYLIP standard
• Human E3 ubiquitin-protein ligase MYLIP detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human E3 ubiquitin-protein ligase MYLIP (MYLIP) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYLIP. No significant cross-reactivity or interference between Human MYLIP and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The ERM protein family members ezrin, radixin, and moesin are cytoskeletal effector proteins linking actin to membrane-bound proteins at the cell surface. Myosin regulatory light chain interacting protein (MYLIP) is a novel ERM-like protein that interacts with myosin regulatory light chain and inhibits neurite outgrowth. E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.

Alternative Names: MYLIP; RP1-13D10.1; IDOL; MIR; band 4.1 superfamily member BZF1; cellular modulator of immune recognition (c-MIR)

Search name: MYLIP; RP1-13D10.1; IDOL; MIR; band 4.1 superfamily member BZF1; cellular modulator of immune recognition (c-MIR)

Tag: MYLIP